Indigenous and synanthropic populations of Galanthus nivalis L. in Baden-Württemberg

Biosynthesis, primary structure and molecular cloning of snowdrop (Galanthus nivalis L.) lectin.

Poly(A)-rich RNA isolated from ripening ovaries of snowdrop (Galanthus nivalis L.) yielded a single 17-kDa lectin polypeptide upon translation in a wheat-germ cell-free system. This lectin was purified by affinity chromatography. Translation of the same RNA in Xenopus leavis oocytes revealed a lectin polypeptide which was about 2 kDa smaller than the in vitro synthesized precursor, suggesting t...

Biosynthesis of the Snowdrop (Galanthus nivalis) Lectin in Ripening Ovaries.

The biosynthesis and processing of the Galanthus nivalis agglutinin were studied in vivo in ripening snowdrop ovaries. Using labeling and pulse chase labeling experiments it could be demonstrated that the snowdrop lectin is synthesized as a precursor of relative molecular weight (M(r)) 15,000 which is posttranslationally converted into the authentic lectin polypeptide of M(r) 13,000 with a half...

Dezentrales Wissensmanagement bei der EnBW Energie Baden-Württemberg AG

Integration von heterogenen Komponenten des Umweltinformationssystems (UIS) Baden-Württemberg

Das Projekt INTEGRAL Integration von heterogenen Komponenten des Umweltinformationssystems (UIS) Baden-Württemberg wird seit Herbst 1993 am Forschungsinstitut für anwendungsorientierte Wissensverarbeitung (FAW) an der Universität Ulm im Auftrag des Umweltministeriums Baden-Württemberg durchgeführt. Das FAW Ulm wird dabei durch das Institut für Kernenergetik und Energiesysteme (IKE) der Universi...

Localization and topogenesis studies of cytoplasmic and vacuolar homologs of the Galanthus nivalis agglutinin.

The Galanthus nivalis agglutinin (GNA) is synthesized as a preproprotein. To corroborate the role of the different targeting peptides in the topogenesis of GNA and related proteins, different constructs were made whereby both the complete original GNA gene and different truncated sequences were coupled to the enhanced green fluorescent protein (EGFP). In addition, a GNA ortholog from rice that ...